FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_40.3520000003901a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_40.3520000003901a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305062
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5100.16717913079964072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCT455.459554E-419.996945
GGTCTAT400.006499378518.7502041
GTCCACA400.006499378518.7502041
GTGTATG755.5986544E-516.0001751
ACCAGAG706.025854E-414.9977053
ACACAGT600.003749322114.99770456
GTATTAC851.6270927E-414.11781
TATACCG750.001016112213.9978575
TCCCATA750.001016112213.9978572
ACCATAG650.00637834213.8440365
TATAGGG650.00637834213.8440365
GAACAGC954.1771622E-412.6296466
ACAGCTA850.002607671812.3510518
ATACTGT1252.563134E-511.9981646
TTCTAGA900.0040001311.6648822
GAACGGC950.005979025711.052752522
GAATTAA950.005979025711.05275251
ATAGTAC950.00598632811.05094053
GAATATA1408.127065E-510.7144021
CTTGAAC1000.00873032110.50355929
Produced by FastQC (version 0.11.2)