FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_39.35200000038fd8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_39.35200000038fd8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559021
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7100.12700775105049722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTAC901.5805812E-514.9980773
TAGACAG1002.5711943E-614.9980765
CTCTGTG750.001017783213.9982047
GTATTAG1804.612957E-912.50622751
TAGGTAG1201.691807E-512.4983975
GTATAAA3100.012.1028021
GGTAGTC1151.5775027E-411.7376267
ACGGTGT1151.5775027E-411.7376268
TAATACT2850.011.5774624
TAGTCAG950.005995798411.0512149
CGGTGTT1100.001432218610.9076939
AGGATAG1100.001432218610.9076935
TGTAACA1100.001432218610.9076932
TTAGGTA1807.855415E-710.8319444
CTAATAC1807.855415E-710.8319443
TATTAGG1704.668451E-610.5868782
GACATAG1000.00874689610.50335130
CTATAAG1451.1581062E-410.3499821
AACAGTT2053.7607788E-710.2425897
CGGCATA1200.002946387310.00140225
Produced by FastQC (version 0.11.2)