FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_38.35200000038f95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_38.35200000038f95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622956
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8730.14013830832354132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTATA550.00210124116.3624112
ATTACAC1102.3565008E-816.3610973
TTTAGGC600.003755226314.9976723
TTATGCC650.006388284713.8440053
ACTGTCG650.006388284713.8440058
GGTCTAT954.1654822E-412.6377341
TATACTG1456.8916415E-712.4118665
GTATAAA1702.7974238E-812.358961
GTACTAA1006.415336E-412.0058481
GTATTAA1501.0303611E-612.0058471
GTATTAT1406.2667514E-611.7914571
TCTTATA1406.3061434E-611.7848312
GTCTATA1151.5772758E-411.7382512
CTTATAC1151.5784551E-411.73730853
CCTTAAG900.003989014311.6723521
TATACCC1059.7124936E-411.4267983
TAATACA1859.141331E-811.349594
TAAAGGT1602.2594868E-611.2482554
ATAGGTG1202.344205E-411.2482545
ATAACAC1355.6465302E-511.1093863
Produced by FastQC (version 0.11.2)