FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_37.35200000038f52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_37.35200000038f52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890140
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15420.17323117711820613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCT350.003008535121.42500110
GTGGACT1501.3607869E-510.9981674
GTATTAG1653.2545722E-610.912791
ACTAAAC1551.9561288E-510.6433883
CTATACT2402.6520866E-910.6232294
GTATAGG1000.00875244310.503561
TTCGCCA1200.002944457610.00339127
ACAGTTC2157.2452167E-79.7658148
CCACAAA1552.2873796E-49.67635113
ACTGAGT1552.2886728E-49.6758078
TAAGACA1552.2886728E-49.6758074
GGGTAGA1409.674709E-49.6461271
ATAATAC4200.09.6412513
GTAAGAC1409.71963E-49.641253
CTATCCT1250.00414449.59844
CTAAACT2209.91582E-79.5438644
GTATGTA2054.142783E-69.515421
GGTATAC2054.142783E-69.515421
TGTACAG1757.426598E-59.4270015
GTCTTAT1603.132434E-49.3781791
Produced by FastQC (version 0.11.2)