FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_36.35200000038f2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_36.35200000038f2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465903
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7280.15625570129404617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG851.625107E-414.1228021
TTCTTAC1056.710029E-512.8618371
GTGTAGT1006.416565E-412.0043821
GTAGTAT1252.556353E-512.0043811
TATATAC2301.05501385E-1011.7371153
CTTATAC1059.705806E-411.4266093
TGTATAC950.005995200511.0507343
TCCTGTA1100.001430680510.9083872
TCTTATA1551.9484187E-510.6444752
CCTTCTA1000.00876811710.4993252
CTTACAC1304.910341E-410.3828333
GTATAAG1602.753078E-510.3162651
GTAATAA1356.915382E-410.0036521
CTGTTCA1552.2835136E-49.6757579
TATACTG1409.701721E-49.6412015
TTGTACA1450.00133589899.3097452
TTATACT1450.0013372099.3087464
TATTATA1851.3558325E-48.91834452
TATAAAA1851.3558325E-48.91834452
GATATAT1350.0077607298.8921351
Produced by FastQC (version 0.11.2)