FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_35.35200000038ee6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_35.35200000038ee6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760244
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20810.11822224646128604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGGC850.002616927512.3506016
CTATACT4650.011.6107044
CCTGGAC950.0060059411.0508523
TAGGACA1807.8982157E-710.8312814
CTTATAC4950.010.3013733
GTCTTAG2802.9831426E-1010.18184951
GGGTTAC1200.002947314610.0032211
TATTATA7200.09.7906462
GTCCTAT1250.00413110069.6030921
TCTAGGC1250.004147619.5984543
TAGACAG3152.5283953E-109.5220075
GTATAGA3950.09.4967291
TAGCCAG1603.1530042E-49.3732255
ACTATAC3051.4224497E-99.3427583
TATACTG4350.09.3085825
GGTATAT3552.5465852E-119.2987691
TCTATAC4700.09.253833
GTATTAT5400.09.1696191
CTTAGAC2957.948984E-99.151073
GTATAAA6650.09.0254621
Produced by FastQC (version 0.11.2)