FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_34.35200000038ea3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_34.35200000038ea3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746954
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13830.1851519638424856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC750.001018956913.9970953
GTCTAGA1151.0895454E-513.0460111
GTATAGA2255.456968E-1212.66912651
GGGTGGT954.1917004E-412.6289597
AAACTGC850.002614850612.3503786
TTAGACT1702.8281647E-812.3503784
ATACTGC1754.2347892E-811.9975096
TTAAACT2103.7380232E-911.42624
ATACAGG1859.1587935E-811.3489966
TAGACTA1755.4765405E-711.1405455
AGTAGAC1355.6530305E-511.1088053
TATACCG950.00600253911.0503395
TCTATAC2903.6379788E-1210.8598163
GTATTAA2504.4929038E-1010.8020971
TTACACT1704.6797177E-610.5860394
GTATTAT2701.5279511E-1010.5576051
GTATAGG1150.0020699410.4368091
CTATATC1602.7766522E-510.310363
TTATACC1602.7766522E-510.310364
TATTATA3550.010.139422
Produced by FastQC (version 0.11.2)