FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_33.35200000038e6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_33.35200000038e6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274210
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCTA455.459594E-419.9958679
GTCTTAT400.006485382618.7563861
TTATACA500.001106119618.0028482
ACACCAT500.001108831617.996286
GTGTAGA859.122696E-615.8877611
CTTCGTA650.00637844313.8432929
ACCATGT650.00637844313.8432928
AACCAGC650.00637844313.8432926
GAGAGCC650.00637844313.8432927
TAACCAG650.00637844313.8432925
GTATATA1106.839442E-613.6410071
TATTAGA1106.852357E-613.6385192
CTTACAC1004.248299E-513.4996733
ATACTAT800.001653385613.1222886
TATATTG1056.726411E-512.85448555
TTACACT1056.726411E-512.85448554
AGTACTG850.002607560512.3503885
TACACTT900.003999969511.6642565
TATACTA1059.686389E-411.4262095
CCTAACA950.005969865211.0543792
Produced by FastQC (version 0.11.2)