FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_32.35200000038e2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_32.35200000038e2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664683
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10030.1508989999744239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCTC601.8656136E-417.4982458
GGGCTGA706.036706E-414.9984956
GGCTATA851.6207153E-414.1289861
CTAGTAG851.6331994E-414.1162323
GTCTAAG650.006348454413.8572761
ACGACAG650.006386555313.8447665
CTAAGCA1004.269225E-513.4986464
ACCTAAT1803.2196112E-1013.3440441
TAGGTAG800.001656379213.1236825
TGTGTCT800.001656379213.1236828
CTAATAC1753.0631782E-912.8558533
GGTCATA954.155051E-412.6417261
CTAGAAC954.1868322E-412.6303123
CTGATCC954.1868322E-412.6303129
TCTATAC2051.9281288E-1012.4377773
TGCAGCC850.002610752112.35263212
ATAGCGT850.00261222612.3517038
ACCGTCG850.00261222612.3517038
GATTAGA1354.162701E-612.232041
TTATAGT1857.003109E-912.1609424
Produced by FastQC (version 0.11.2)