FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_31.35200000038df4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_31.35200000038df4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533932
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26170.17060730201860316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC951.5126152E-615.786668
GCGAGTA600.003746925615.00466329
CTAATAC4050.013.3309563
GTATTAG3850.013.2487121
CGCGCCT850.00261260712.35315722
GTGTTAA3050.012.2969011
GCACCGC1101.04539344E-412.270146
TAGGTAG1652.5621557E-711.8160755
GGGGCTC900.003997854411.67067530
CGCTCCT900.00399980311.66991326
GCGCGCC900.004006630711.66725121
CCTATAC2850.011.5768843
CCCTAAG1303.8396436E-511.5401671
GTCTTAG1602.2583372E-611.2516631
TAATACT7050.011.0618574
CTAGGGC950.00600611411.0506623
TAGGACT1100.001435289610.9071464
TAAGACA2905.456968E-1210.8601334
CGATCAC1253.4170394E-410.80194924
TACACTG2504.5474735E-1010.7980765
Produced by FastQC (version 0.11.2)