FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_30.35200000038db0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_30.35200000038db0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676681
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31470.18769223245208838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1051.3320459E-817.1401823
ACCGCGC600.003758777914.997668
CCTAGGT800.001658907713.12295152
AGCGCAC1456.914979E-712.41185610
CCGCCGT850.002608587712.355803525
CTTAGGG850.002616185512.3510133
AGGGGTG900.004013070411.6648455
GTATTAG4650.011.6183671
GTCTAGG1303.827514E-511.543891
AGGACGG1100.00143514510.9073895
TAATACT7400.010.7415674
TAGACTG1408.19128E-510.7126145
GCCGCGT1150.002071608310.43720125
GGCTATA1756.5773766E-610.2905531
GTCTAGA1901.5691785E-610.2679871
CTAAGAC2352.1702363E-810.2111723
GTTTAGG1501.6362134E-410.0047041
CAGCGTC1200.00295888949.998449
ACAGCGT2251.2572673E-79.998448
CTGTGCC1200.00295888949.998449
Produced by FastQC (version 0.11.2)