FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_29.35200000038d7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_29.35200000038d7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597478
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17500.10954767452196525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4550.013.5214651
TAGGTAG2700.012.7752855
CTGATCG1252.5802614E-511.997669
CCCTGCC1151.5820081E-411.7368399
CGTCGTG900.004000040711.66986930
CCTGCCC900.004013161611.66475610
CCGTCAG900.004014100411.6643919
GGTAGTC2701.8189894E-1211.6643917
TATACTG6350.011.336375
TAGTCAG2659.094947E-1211.3185469
TGGACAC1501.3630941E-510.9978545
GCCGCAG1100.001433299610.90901217
ACTGCGC1000.00879230410.4979518
CGTGAGC1150.002076718310.43405212
TAATACT8350.010.4171294
ATACTGT6300.010.2360966
TTATACT6400.010.07615954
GTGGCGT1501.63934E-410.00274630
CCGCAGT1356.9567614E-410.0002418
ACGTGAG1200.00295741489.99898811
Produced by FastQC (version 0.11.2)