FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_28.35200000038d3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_28.35200000038d3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363337
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23660.17354476552752549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG400.006519934618.7455983
GCACTGC809.77381E-514.9964786
GAGTCCG902.6577868E-413.3302049
CTGTCCC1151.0959486E-513.0404179
ACACCGC1056.766415E-512.8541258
GTATTAG4500.011.6690641
CGATCAC1303.833782E-511.54167824
GTGTAGG1059.696114E-411.4309211
ACTGATC1501.3632864E-510.9974188
GACAGTC1100.001435789410.9065297
AGAGTCC1551.9597062E-510.6426638
ACCTAGC1000.00875321210.50408529
TAGACCG1000.00879377610.4975355
CCTGGAC1150.002079230310.4323333
GAGAGTC1304.9262313E-410.3821777
GACTGTG1304.9262313E-410.3821777
AACAGCG1304.9262313E-410.3821777
ACAGCGC1451.16954994E-410.3423998
TCCTAAG1200.00295841469.9983862
GTATAAT7100.09.9316181
Produced by FastQC (version 0.11.2)