FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_27.35200000038d03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_27.35200000038d03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706650
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24490.14349749509272552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2000.016.497475
CGGGCGG600.003756549714.99901911
CGACGGG600.003758747114.99778
GACGGGC600.003758747114.99779
GTATTAG3800.013.0319561
GACCCTA1006.460112E-411.998167
GGTAGTC1901.3279532E-711.0509377
GTATTAA5900.010.9369361
TATACCG3250.010.6137565
GACCCTC1000.00878999310.498397
CTAGTCC1000.00878999310.498393
TACACCG1901.5818459E-610.2615845
CCGGATC1356.9480174E-410.0016925
TATTAGG5250.09.99846652
TACACTG3001.0004442E-109.9984665
GTACTAG3051.3460522E-109.8403231
CCCTTAT2752.3501343E-99.82243251
GGATAAG2301.717508E-79.7868431
TACGAGT1409.728098E-49.6413784
TTAGACC2054.1771564E-69.5107364
Produced by FastQC (version 0.11.2)