FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_26.35200000038cce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_26.35200000038cce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163386
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19450.16718440827034192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG455.477868E-419.9938987
GACGCGC559.601931E-519.0850838
CGGCCGA400.00651232118.74911315
CGCGCAT500.001111547917.99682810
CCTAGCG1001.4165744E-716.50631530
GCGCACT550.002102932216.36215811
GGTAGTC1750.016.2807457
CGATCAC1002.5667869E-615.00380624
TAGACGG1002.5817408E-614.9954225
TAGGTAG1709.094947E-1214.9954225
TGACGCG600.003761587214.9954227
TAGTCAG1503.0559022E-1014.9954229
CTAGGCA1301.8328319E-713.84371410
GTGACGC650.00639903313.84192856
ACGCGCA650.00639903313.84192859
CTATGCA2550.013.5252839
GTATTAG3400.012.7962631
CCTAATA3700.012.1589742
TATTAGG3250.011.9968542
CTAGACA1252.5817551E-511.9963394
Produced by FastQC (version 0.11.2)