FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_25.35200000038c8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_25.35200000038c8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555750
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12030.21646423751686908No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCA651.648093E-518.4554485
ACAGTGC752.7997175E-617.9940628
TAGCACA550.00210444116.3582384
GTATTAG1052.467823E-715.7161711
CTACACA809.765163E-514.9950524
TAATACT2300.014.9950514
TAATACC1104.204121E-714.9950514
CGTGAGC600.003758914514.99505112
TTACCCC600.003758914514.99505110
ACCCCAG600.003758914514.99505112
CCTAATA1351.8495484E-814.4422782
TGACCAG750.001019322513.9953825
CCATACT750.001019322513.9953824
GTATTAA1301.82039E-713.84781551
GTATAAG1301.82039E-713.84781551
CCGTAAG650.00639453813.8415869
ACAGCGT650.00639453813.8415868
CTATGCA1004.275979E-513.4955469
TAGGTAG902.6554923E-413.3289365
CAGTGCA902.6554923E-413.3289369
Produced by FastQC (version 0.11.2)