FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_24.35200000038c48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_24.35200000038c48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600932
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15410.256435004293331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC559.5712836E-519.09048524
CCCCCGA400.006510925518.74802415
TAGGTAG703.1112417E-517.141055
GTTAGAC703.1112417E-517.141053
CCGACCG550.002101553116.36191218
TTAGACT653.4359598E-416.1521434
TAGTCAG653.4359598E-416.1521439
CCCCGGC755.6187637E-515.99831423
GTATTAT1850.015.4153251
AGACTAG600.003753776414.9984197
AGCCGGG706.035745E-414.99841915
CAGCCTG600.003753776414.9984199
GGCGTCG600.003753776414.9984198
GTAATAC706.035745E-414.9984193
GTAAGCT706.035745E-414.9984194
GTCGCTT851.6328588E-414.11615911
ACTAGAC750.001017763213.9985253
CCGAGGC750.001017763213.99852513
TTAACCC750.001017763213.9985254
TCAACCC1402.9833245E-813.9271033
Produced by FastQC (version 0.11.2)