FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_23.35200000038c04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_23.35200000038c04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510064
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19640.13006071265853633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2750.016.9047055
CGGGTCC859.18349E-615.88664925
TAGTCAG2650.015.8449469
TAGACTG1401.8590072E-914.9961095
GGTAGTC3050.014.7502717
TTAGGTA3700.014.5908094
GTATTAG5050.014.2617561
ATTAGGT4850.013.6047183
TATTAGG5000.013.4969462
TCGGGTC1352.88901E-713.33694124
GTCTAGT1151.0900705E-513.047441
GGACAGG954.197946E-412.6283036
AAGCCCG850.002618005512.3497365
ACCTAGC1151.5731133E-411.74386329
CTAGTAC2351.546141E-1011.4863823
TCTATAC3750.011.1970943
TAGGACA1807.9025085E-710.8305234
GTAGTCA3850.010.5167518
TTGCGCG1000.00877455310.50075319
TGGTCCG1000.00879396210.49762414
Produced by FastQC (version 0.11.2)