FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_22.35200000038bcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_22.35200000038bcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712925
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13560.1902023354490304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGC859.2053015E-615.88019858
GTACAGG951.5058686E-615.7906551
GGTAGTC1052.477882E-715.7121547
CAGGGCC600.00375544514.9979669
GACTGAG600.00375544514.9979667
GTCTTAG750.001016703814.0010481
GTCTTAT1106.8952468E-613.6373841
ACAGCGC800.001657024513.123228
CTAATAC2650.012.4511413
TAGTCAG850.002613240212.3512659
TTAGGTA1354.2078154E-612.2205654
CTATACG900.004008590711.6650864
TAGGACA900.004008590711.6650864
TGCGAAA1059.708204E-411.42782312
GTCATAC1059.713909E-411.4270223
TATACAG2555.4569682E-1111.1749545
GTATTAG2155.3787517E-911.1636261
GTATAGA2853.6379788E-1211.0534581
ACTGCTC950.005998872711.0511338
CTGCCCA950.005998872711.0511339
Produced by FastQC (version 0.11.2)