FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_21.35200000038b8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_21.35200000038b8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119261
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCC200.005131447729.9970653
TCTGGAC200.005131447729.9970652
CTGGACC200.005131447729.9970653
CTATACT402.4511883E-422.4977994
TTGGACT350.002992260721.4264744
GAATAAT455.4307864E-419.9980451
ATTATGC455.4307864E-419.9980453
GTCTGAA400.00648078618.7481651
ACCGCGT400.00648078618.74816517
TTATACT400.00648078618.7481654
AACTCCG400.00648078618.7481657
CTTACAA400.00648078618.7481652
ACCAGCT550.00208233316.36889830
AACCAGC650.006328413.85060629
Produced by FastQC (version 0.11.2)