FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_20.35200000038b49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_20.35200000038b49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322908
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6570.2034635252146122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCA300.001226378124.9939379
CTGTCTG350.003005755421.4233749
CTAATAC859.586074E-1021.1713333
TCCTAAG504.592585E-520.9981582
TCTATAC651.643374E-518.457063
GAAGTGT500.001109679817.9956326
AATACTA1152.2082531E-916.9524085
GCGAAAC805.1542265E-616.8735213
AGAAGTG908.5572174E-716.6626245
GGGACTG550.002094131216.3672729
ATTACCC550.00210064516.3596673
TAGCCTG550.00210064516.3596675
TAGAAGT653.433393E-416.1499274
TAATACT1401.07320375E-1016.067534
CTAAGCC755.612443E-515.99611953
GTATAGA859.143034E-615.8858791
CACAGCC706.0100673E-415.00333120
CACCTTG1002.5619647E-614.99868414
TATACAC809.737756E-514.9963633
ATAGCCT600.003752191314.9963624
Produced by FastQC (version 0.11.2)