FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_19.35200000038b05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_19.35200000038b05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600608
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26420.1650622763349927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG455.477025E-419.9950249
GGTAGTC2900.017.0647167
TAGTCAG3000.016.995779
TAGGTAG3500.016.2816625
CGACCCC600.003760218914.99673610
GTATTAG5200.014.7175311
TATTAGG4500.014.6648482
CCTAGTC1251.1353768E-714.3982152
GTACTAG2300.014.3536721
TTAGGTA4350.013.7896714
ACCCGGA1651.2732926E-913.638082523
TCAGATG3700.013.37671912
CGACGGG1251.7826424E-613.1967158
CTGCGCA800.001660009813.1217349
GTAGTCA3900.013.07366858
CGGGCGG1404.51686E-712.85554911
GTGGCGT1907.694325E-1012.6355430
GTAGGGC850.002617916312.3498688
CCGCCCG850.002617916312.3498689
CTATGCA3650.012.3256999
Produced by FastQC (version 0.11.2)