FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_18.35200000038ac0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_18.35200000038ac0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901890
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35950.18902249867237328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC2700.016.1080477
TAGGGGT1052.4857763E-715.7112974
CTAATAC6250.014.3972633
TATTAGG5300.013.5830362
TAGGTAG3100.013.5458115
TAGTCAG2800.013.390319
CCTAATA5300.013.3000562
CTATGCA4550.013.1843059
GGCGCCG1601.20926416E-813.12802625
GTATTAG5300.012.4540631
ATTAGGT4700.012.4444423
GCCCCCG1101.0444205E-412.27168414
ATCACAC3950.011.77517529
ACTATGC6500.011.7669938
ACCTAGC2052.5538611E-911.71032429
CCGACCG900.0040081311.66690218
CCCCCGA900.004009705511.66628815
GTATTAA4900.011.6338051
AGACTAG2103.754394E-911.4263987
CCTAGCG2259.076757E-1011.33684130
Produced by FastQC (version 0.11.2)