FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_17.35200000038a8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_17.35200000038a8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431383
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15470.10807729307949025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2250.018.6630155
TAGTCAG2150.016.7409119
ACCTAGC1154.0574378E-815.65841129
GGCCGAG600.003759409814.99706612
GGTAGTC2300.014.9970667
GTGCGCG706.047115E-414.9970659
CCTAGCG1251.1278644E-714.40573830
CCGTCGC1156.962491E-714.3450199
CTATGCA3700.014.1864149
GTATTAG4350.014.1391161
CGATCAC1504.8767106E-914.00460124
GTCTACG902.6507737E-413.3344521
CCTAATA4950.012.7256722
TCACACA3450.012.6137230
ATCACAC3600.012.08814829
TACTGTG2257.2759576E-1111.9976527
AGTCAGA2900.011.89422510
CTAATAC5850.011.7925643
TAATACT8650.011.78964
CTGATCG900.00401376611.6643859
Produced by FastQC (version 0.11.2)