FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_16.35200000038a4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_16.35200000038a4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593726
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27080.16991628422953506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4300.016.751091
TAGGTAG2600.016.1508735
TAGTCAG2550.014.1150489
GGTAGTC2900.013.4458017
TATTAGG5450.012.1093832
ATCACAC3550.011.83400629
TTAGGTA4450.011.7955824
AGTCAGA3700.011.75459310
CTAGGAC1151.5818405E-411.736973
CTATGCA4350.011.721989
CTGTCCG900.004013763311.6645199
ACTATGC5650.011.4138288
CTAATAC5950.011.3424493
CCTAGCG1355.633935E-511.11429630
ATTAGGT4750.011.0505973
TGGCGAC1100.001429784810.91221930
TAATACT8100.010.7387654
TAGACTG1408.193026E-510.7123145
GTAGTCA4100.010.6078038
TCACACA4000.010.5030130
Produced by FastQC (version 0.11.2)