FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_15.35200000038a13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_15.35200000038a13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617424
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21890.13533866197113434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGT1001.4195211E-716.50433725
TCGCCGC1301.8293576E-713.84679723
GCCGGGT650.00639472813.84380111
CGCCGCG1201.1197535E-613.75361424
TCACACA3000.012.5040630
GTCCTAG1101.04205035E-412.2744351
CCGCGTC1406.2998697E-611.78881326
CCCTAAG1303.8402846E-511.5400681
TCTACAC3000.011.4980453
TCTATAC5000.011.3980633
ACCGCGT1459.33521E-611.37948617
TAGGTAG2402.237357E-1011.2480875
AGCGCAC1501.3627443E-510.99813110
CTAATAC5200.010.9596753
GTATAAA7550.010.9286741
CAGCGTG1253.428351E-410.7981649
ACCTAAT5450.010.7354381
GTATTAG5600.010.7157761
ACGGTGT3500.010.7124638
TATACGG5050.010.6912515
Produced by FastQC (version 0.11.2)