FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_14.352000000389c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_14.352000000389c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479967
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24580.16608478432289367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT859.211277E-615.8815484
TAGACTG1557.77618E-913.5472435
GTCTTAG1652.5353438E-711.8255741
GACGGCC900.004011556511.6652877
GTATTAG3400.011.4777631
CCCTAAG1059.662936E-411.43571951
TTAATAC4900.010.7137423
CGCGAAT1000.0087867810.49875810
GTCTAAG1901.5655969E-610.2695771
GTATATA12100.010.1716481
GTATTAA3850.010.1362061
GTGTAGA3152.3646862E-1110.0062551
GTATAAT7650.010.0062551
GTTCTAT3357.2759576E-129.8569071
GTCTAGT1409.6573593E-49.648891
CTAAGAC1409.71734E-49.6423683
AGACCAT2651.3513272E-89.6215046
TGTGCGA1250.00414548869.59886510
GCACTGC1250.00414548869.5988656
AGAACTG3306.002665E-119.5446475
Produced by FastQC (version 0.11.2)