FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_13.3520000003898e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_13.3520000003898e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769934
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31770.1794982185776419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCGC650.00637856513.84919328
CGATCAC1106.898774E-613.63935724
CTAGGGT1507.4060154E-812.9977114
GTATTAG4600.012.72090151
TAGGTAG3100.012.578435
TATTAGG5200.011.8251742
CTAATAC5150.011.0660123
CTCGCAA950.00598898211.05505827
ATTAGGT4000.010.8730863
CCTAATA5250.010.5698662
ACAGGGG1000.00879169110.4981528
GTCTAAT2301.5434125E-810.4376631
CGCGAAT1150.002077278410.43382914
ATTATAC7050.010.2109673
AGTCAGA2957.2759576E-1110.16827510
ACCTAAT5350.010.0962441
CAGCGTG1200.00295951929.99823959
GGTAGTC2854.1472958E-109.99823957
ACGGTCC1200.00295951929.99823958
TACACTG2752.3756002E-99.8164535
Produced by FastQC (version 0.11.2)