FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_96.35100000039e46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_96.35100000039e46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650395
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT39780.6116283181758777No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC28560.43911776689550197No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC27880.42866258196941853No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG22350.3436373280852405No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC22290.34271481176823315No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT20800.31980565656255044No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC20310.31227177330699035No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG19310.29689650135686774No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC17080.2626096449080943No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT16680.2564595361280453No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT14820.22786153030081718No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG14260.21925137800874853No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG14250.21909762528924728No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG13700.21064122571667984No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG13640.20971870939967252No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG12860.19772599727857684No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT12470.19172964121802905No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT12390.19049961946201924No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC12100.18604079059648368No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC11970.18404200524296774No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC11670.17942942365793094No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC11500.1768156274264101No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT10840.16666794793932918No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC10670.16405415170780835No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT10460.16082534459828257No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT10250.15759653748875685No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA10230.1572890320497544No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT9910.15236894502571513No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA9650.14837137431868325No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA9120.14022248018511826No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT8900.1368399203560913No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC8830.1357636513195827No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC8630.13268859692955817No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG8510.13084356429554347No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT8460.13007480069803734No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG8410.1293060371005312No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT8290.1274610044665165No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG8150.12530846639349932No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC8020.1233096810399834No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT7930.12192590656447237No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT7920.12177215384497114No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT7830.12038837936946009No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA7680.11808208857694169No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7660.11777458313793925No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA7570.11639080866242821No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA7460.11469952874791473No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG7440.11439202330891228No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7440.11439202330891228No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC7370.1133157542724037No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA7350.11300824883340124No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC7340.11285449611390001No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC7290.11208573251639388No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA7280.11193197979689265No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC7280.11193197979689265No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7090.10901067812636937No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA7020.10793440908986078No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC7010.10778065637035955No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG6940.10670438733385097No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG6850.10532061285833992No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA6730.10347558022432521No Hit
GAGTTAGCCGGTGCTTATTCTGTCGGTAACGTCAAA6600.10147679487080927No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA6550.10070803127330316No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC6510.10009302039529824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAT452.0396306E-523.33223
CGTAAGG452.0396306E-523.33222
ACTCGGT1250.022.798893
TGTAGGA603.2839307E-722.4989032
GTGTAGG553.882429E-621.817121
CCGTATG7200.021.457298
TATGCGC3850.021.42752811
CTCGGTC1350.021.1100834
ACCGTAT7250.021.102427
TAGGATA504.600414E-520.9989764
CGTATGC7400.020.8773619
GTATGCG7550.020.4625810
CCACCGT7700.020.453555
GGGGTCT608.245679E-619.9990258
TCTAGCC701.4442703E-619.28477520
TATGCGT3750.019.19906411
GAGCTAA559.5747746E-519.0899811
CACCGTA8350.018.8613576
CATCCAC7900.018.7965552
GGATACA802.4877409E-718.75341229
Produced by FastQC (version 0.11.2)