FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_95.35100000039e02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_95.35100000039e02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891103
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT71830.8060796563360241No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC45090.5060021119892987No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC35470.39804601712708854No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG31810.35697332407140364No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG25580.28705996949847545No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT25310.2840300167320725No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG24740.2776334497807773No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC24710.2772967883622881No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC24490.2748279379600338No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT23650.2654014182423356No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG23070.25889263081821073No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG21460.24082513469262254No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC21110.23689741814358162No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT20530.2303886307194567No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG20060.22511426849645888No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT19180.21523886688744176No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC19160.21501442594178227No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC17870.20053798494674577No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG17780.1995280006912781No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA17710.19874245738146992No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC17380.1950391817780885No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT16660.1869593077343472No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC16570.18594932347887955No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT15130.16978957539139694No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT14930.16754516593480215No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG14730.16530075647820736No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC14500.1627196856031233No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC14230.15968973283672033No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT13850.1554253548691902No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT13570.15228318162995746No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT13270.14891656744506526No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG13120.14723326035261916No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT12710.1426322209665998No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA12490.14016337056434552No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT12180.1366845359066236No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA12170.13657231543379386No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG11970.13432790597719904No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA11900.13354236266739086No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG11900.13354236266739086No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT11830.13275681935758268No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG11690.13118573273796633No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA11670.13096129179230684No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC11610.13028796895532838No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA11560.12972686659117968No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC11550.12961464611834997No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG11410.1280435594987336No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT11390.1278191185530741No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA11360.1274824571345849No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC11090.12445250436818191No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA10940.1227691972757358No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA10880.12209587443875736No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT10770.12086144923763023No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT10480.11760705552556776No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT10230.11480154370482425No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG10180.11424044134067556No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC10150.11390377992218632No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC9790.10986384290031567No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA9690.10874163817201828No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA9650.10829275628069931No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC9620.10795609486221008No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG9540.10705833107957217No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT9520.10683389013391269No Hit
CTCCTCTACCGCATCATCAAAAGATGATACCCGTAG9460.10616056729693425No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC9300.10436503973165841No Hit
CGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACC9140.10256951216638256No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT9110.10223285074789333No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC9050.1015595279109149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT254.227522E-429.9976441
ACCCTAG300.001226899924.9980376
ACTCGGT2550.024.1157533
CTCGGTC2750.022.9072914
CCTCTGA1450.022.75683413
GTCTCTT804.5110937E-1022.50454530
GTCCGAA603.2877142E-722.4982342
GCCTCTG1750.021.4268912
TACCCTA350.003007000321.4268885
GCCCTCG350.003007000321.42688811
TAGGGCC350.003007000321.4268884
CTTTCAT1700.021.1748074
GAGCCAT4950.020.604441
CGTCTTT1750.020.5698131
CATACCC12250.019.8351763
TCTGACT1600.019.68595515
CCATACC12350.019.553122
GCCATAC12500.019.4384731
ACCGTAT9500.019.4195277
CCGTATG9750.019.3830958
Produced by FastQC (version 0.11.2)