FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_94.35100000039dcd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_94.35100000039dcd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630535
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT48340.766650542793025No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC22110.3506546028372731No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG18580.29467039894692604No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC18440.2924500622487253No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG17750.2815069742361645No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG13560.21505546876858542No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT13400.21251794111349886No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC13220.2096632225015265No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG13190.20918743606619775No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA12730.20189204405782393No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG12640.2004646847518377No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC12600.1998303028380661No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG12580.19951311188118026No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT12460.19760996613986537No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT11890.1885700238686195No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC11870.1882528329117337No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT11440.18143322733868858No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT10570.16763542071415544No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT10220.1620845789686536No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC9990.15843688296446667No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG9880.15669233270159466No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT9330.14796958138723465No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8960.142101548684847No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC8780.1392468300728746No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC8770.13908823459443173No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC8630.13686789789623097No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG8540.1354405385902448No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT8410.13337879737048697No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG8410.13337879737048697No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8200.13004829232318588No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC8070.12798655110342805No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT8040.12751076466809932No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT7920.12560761892678438No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT7880.12497323701301276No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG7800.12370447318546948No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA7500.1189466088321822No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA7490.11878801335373929No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC7480.11862941787529636No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT7310.11593329474176692No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA7290.11561610378488109No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT7260.11514031734955235No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC7220.11450593543578073No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7100.1126027896944658No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG7030.11149262134536544No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC6990.11085823943159381No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC6760.10721054342740688No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG6710.10641756603519234No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC6680.10594177959986362No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT6660.10562458864297779No Hit
CTTCTAAGCCAACCTCCTAGCTGTCTAAGCCTTCCC6320.10023234237591885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTAT200.00514651529.9988121
GTCTCTT551.4153738E-724.54837430
GATTAGA804.5110937E-1022.499112
TACCCTA350.003005357221.4277215
CCATACC8800.021.1355252
GCCATAC9050.020.883151
ACCGTAT4600.020.8687387
TACCCTT8650.020.635025
CATACCC9100.020.6035793
TATGCGC3500.020.57061411
CCGTATG4700.020.424728
GTACTAC751.2637611E-719.9992081
TTAGATA901.9608706E-919.9992074
CCACCGT4950.019.6961885
ATACCCT9900.019.2416614
ATCCACC5200.019.0377063
GTATGCG5050.019.00914610
ATTAGAT953.8380676E-918.9466173
AACCCAT400.006508802518.7492586
ATGTACT400.006508802518.7492584
Produced by FastQC (version 0.11.2)