FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_93.35100000039d8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_93.35100000039d8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70280
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT3310.470973249857712No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC2510.35714285714285715No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2500.35571997723392146No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2180.3101878201479795No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT1640.2333523050654525No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1370.19493454752418896No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1340.1906659077973819No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG1330.1892430278884462No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG1300.18497438816163914No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1200.1707455890722823No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1180.16789982925441094No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1130.1607854297097325No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC1130.1607854297097325No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG1120.1593625498007968No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1070.15224815025611838No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC1050.14940239043824702No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT1050.14940239043824702No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT990.1408651109846329No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT990.1408651109846329No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG980.13944223107569723No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG930.1323278315310188No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG900.12805919180421174No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC890.12663631189527602No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG860.12236767216846899No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC780.11098463289698349No Hit
CCCATTATACAAAAGGTACGCAGTCACCCAACAAAG750.10671599317017644No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA740.10529311326124076No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA730.10387023335230507No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC720.10244735344336939No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC720.10244735344336939No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA200.005114810529.9999982
CCGGTCT559.417423E-519.090919
CGGAAAT400.00645124318.74999825
GTCCATG400.00645124318.7499981
CAAGAGT500.001096183318.014
TCGACGG500.001096183318.026
GAGTTCA500.001096183318.017
AGAGTTC500.001096183318.016
TCCGACC500.001096183318.04
CATATCG500.001096183318.022
TCATATC500.001096183318.021
CCCAAGA550.002075333816.36363812
CCGGTCG550.002075333816.36363820
GGTCTGA653.379544E-416.15384711
CGGTCGG653.379544E-416.15384721
GCCCAAG600.003707403815.011
GATACCG705.937487E-415.05
TTCCGAC600.003707403815.03
AAGAGTT600.003707403815.015
TATCGAC600.003707403815.024
Produced by FastQC (version 0.11.2)