FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_92.35100000039d47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_92.35100000039d47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129204
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT7310.5657719575245348No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3570.27630723507012167No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC3430.26547165722423455No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC3080.23838271260951677No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3030.23451286337884278No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3000.23219095384043836No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG2640.20432803937958577No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2530.19581437107210306No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG2480.19194452184142907No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2450.18962261230302468No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2040.1578898486114981No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG1970.15247205968855454No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1950.15092411999628494No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1920.14860221045788055No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1900.14705427076561098No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1770.13699266276585864No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG1760.13621869291972385No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1670.1292529643045107No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1660.1284789944583759No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1640.1269310547661063No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1640.1269310547661063No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1620.12538311507383673No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1610.12460914522770195No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1590.12306120553543234No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1540.11919135630475838No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1540.11919135630475838No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1500.11609547692021918No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1490.1153215070740844No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC1370.10603386892046687No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC1370.10603386892046687No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG1370.10603386892046687No Hit
CTCCTCTACCGCATCATCAAAAGATGATACCCGTAG1370.10603386892046687No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1360.10525989907433206No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG1350.10448592922819727No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1330.10293798953592767No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1320.10216401968979288No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC1300.10061607999752331No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC1300.10061607999752331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAT359.909352E-525.71395325
TGCGCAC851.8189894E-1224.70556313
TATGCGC801.6370905E-1124.37468511
GGTCTAT501.672819E-624.0089841
CCGTATG1150.023.4779598
TATTCCC603.2408207E-722.499715
AGCTATC402.4513176E-422.4997126
CCACCGT1200.022.499715
TACAAGC402.4513176E-422.4997117
CGTATGC1250.021.599729
ACCGTAT1250.021.599727
CGCACAA1051.8189894E-1221.42829515
CCATACC1200.021.2497252
GCGCACA1001.4551915E-1120.9997314
GTTACAT504.5626144E-520.9997319
TTACATC504.5626144E-520.9997320
GTATGCG1300.020.76896310
TCCACCG1300.020.7689634
TCTATTC657.001745E-720.7689633
ATGCGCA951.6916601E-1020.5260512
Produced by FastQC (version 0.11.2)