FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_91.35100000039d03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_91.35100000039d03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113705
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT10790.948946836110989No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC4150.3649795523503804No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3800.3341981443208302No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG3400.29901939228705865No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG3150.2770326722659514No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT2990.2629611714524427No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2720.2392155138296469No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG2700.23745657622795832No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2420.21283144980431817No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2380.20931357460094105No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC2320.20403676179587527No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2080.18292951057561233No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC2050.18029110417307945No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1990.17501429136801372No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1940.17061694736379227No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG1860.16358119695703796No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG1810.1591838529528165No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1730.15214810254606217No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1690.14863022734268502No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1640.14423288333846357No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1600.1407150081350864No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG1580.1389560705333978No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1540.1354381953300207No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1530.13455872652917636No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1500.1319203201266435No Hit
GCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAA1440.12664350732157775No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1400.12312563211820061No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1380.12136669451651202No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1330.11696935051229058No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1310.11521041291060198No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC1300.11433094410975769No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT1290.11345147530891342No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1280.11257200650806913No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT1280.11257200650806913No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1240.10905413130469198No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC1240.10905413130469198No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1220.10729519370300337No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA1180.10377731849962624No Hit
GTCCCAAAGTCATGGTTAAGTGGGAAACGATGTGGG1150.10113891209709334No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1150.10113891209709334No Hit
CTCCTCTACCGCATCATCAAAAGATGATACCCGTAG1150.10113891209709334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGGT200.00512174530.007483
GGAACAC200.005132781329.99428411
TAACTTT751.9463187E-1024.0059835
TTCAAAC751.9645086E-1023.99542611
GCCATAC1550.023.2315961
TACCCTT1500.023.0057355
CCATACC1500.023.0057352
CTAACTT804.3837645E-1022.505614
ATACCCT1600.022.505614
ACCCTTG1600.022.4957126
ACTTTTC804.4019544E-1022.4957127
CCGCTAA908.0035534E-1121.6720681
ACCTAAA350.002985386921.4339141
GGTCTAT350.002985386921.4339141
GCTAACT859.331416E-1021.1817513
AACTTTT859.385985E-1021.1724366
GCAAACT859.385985E-1021.17243622
GATGCAA859.385985E-1021.17243619
CGATGCA859.385985E-1021.17243618
TGCAAAC859.385985E-1021.17243621
Produced by FastQC (version 0.11.2)