FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_90.35100000039cdb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_90.35100000039cdb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68181
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCA2170.3182704859124976No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATG720.10560126721520659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAT200.0051180329.99486429
TCTTATA408.055502E-626.2455042
CTCTTAT408.055502E-626.2455041
AATGATG300.001215964124.995725
CTTATAC559.4245275E-519.087643
GACTAGG559.4245275E-519.0876428
TTATACA752.7232054E-617.996924
GCCCACG601.8358027E-417.49700420
CCCACGA601.8358027E-417.49700421
ACTAGGC601.8358027E-417.49700429
CGAGACT601.8358027E-417.49700425
ACATCTC601.8358027E-417.49700410
CCACGAG601.8358027E-417.49700422
CTAGGCA601.8358027E-417.49700430
ACACATC703.0480642E-517.1399238
TCTCCGA550.002066667416.37284913
CATCTCC653.363185E-416.1629411
AGACTAG653.3820898E-416.15108127
CACGAGA653.3820898E-416.15108123
ACGAGAC653.3820898E-416.15108124
Produced by FastQC (version 0.11.2)