FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_89.35100000039c98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_89.35100000039c98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672996
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGG1400.026.79693425
CCGGCCC1400.026.78299513
AGCCCGG1200.026.24538410
GGGCCCG1450.025.86328723
GAGCCCG1250.025.195579
CCCGGAG1450.023.79068817
CGGCCCG1600.023.43511814
CCCGGCC1600.023.43511812
GCCCGGA1500.022.99766316
GCCCGGC1700.022.05494511
CCGGGGC1300.020.77638427
CCCGGGG1300.020.77638426
CGGAGGG1750.019.71374919
CCGGAGG1850.019.45748318
GGAGCCC1700.019.4083528
GCGCCCG559.584757E-519.0875519
GAGGGCC1900.018.9482621
ACAGCGC400.006514368618.7467028
CGCCCGG400.006514368618.74670210
GGCGCCC500.001110883317.9968348
Produced by FastQC (version 0.11.2)