FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_88.35100000039c55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_88.35100000039c55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973228
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGT650.006387873613.8452536
GTCTAAA1601.2114469E-813.12414551
CGTATAC1354.2089578E-612.2214273
ACAGCGT1459.332918E-611.378578
ACTGTAC1602.2613312E-611.2492688
CTAGACT950.00599839111.0519124
TATACCG1408.175603E-510.7135895
CTTAGAC1408.175603E-510.7135893
ACTGGTC1000.0087796910.4993178
TAACTAG1150.00207538110.4341035
TATAATA7500.010.39932252
GGTCTAC1304.9158186E-410.383941
GTATATA6700.010.2978371
TACACAG1901.5774331E-610.2624895
TATACGG2209.0221874E-810.2266075
AGAGTGC1200.00295475859.999356
AGACTGT1653.8931918E-59.9993496
GTATTAT4550.09.8894651
CATATAC4400.09.885723
CTGTACT1705.4027703E-59.7052519
Produced by FastQC (version 0.11.2)