FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_87.35100000039c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_87.35100000039c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157848
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1900.014.9982295
TAGTCAG1251.1337761E-714.3989229
GTCTTAG952.6381034E-514.2088481
TAGGCTG800.001657893413.123455
ACTATGC2550.012.94020758
CTATCCC1252.5756483E-511.9991029
TATACTG4700.011.80711655
GTATTAG3100.011.6115321
GGTAGTC1951.5321348E-811.5375987
TATACCG2103.74348E-911.4272225
TCTAGAC1459.342681E-611.3779673
TATTAGG2950.011.1851212
TAGGGTT950.00600192111.0513275
TTAGGTA2607.8216544E-1110.9602444
ATACTGT4150.010.8425626
CTATACT3200.010.7799774
AGGTAGT2957.2759576E-1210.6771676
GGATGAC1150.002073825810.43535321
TAATACT4750.010.4198234
GTCCATA1304.9197325E-410.3833891
Produced by FastQC (version 0.11.2)