FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_86.35100000039bdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_86.35100000039bdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040728
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA2204.7293724E-1112.2721972
GTATTAG2600.012.11486151
GCCGCGT900.00400362811.66784425
CGCCGCG900.00400506611.66728424
AGTCCGG900.004007942511.666162510
TCTACAC1806.260234E-811.66616253
TAGGTAG1551.5433397E-611.6124015
GTGTACG1059.712956E-411.4280781
TAGACAG1859.1533366E-811.350865
CAGCGCA950.005997890611.0521549
ACAGCGT1100.001433002910.9086198
GACAGTC1253.422119E-410.7995337
ACTCCGC1253.422119E-410.7995338
GTATTAT4800.010.624541
AGCGCAC1000.00877895810.49954610
GTCTAAT1901.5773876E-610.2627131
TACTATG3101.8189894E-1110.16085157
ACTGATC1356.9567276E-49.9995678
TACACCA2301.7262937E-79.7821865
GTTATAG2301.7262937E-79.7821861
Produced by FastQC (version 0.11.2)