FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_85.35100000039b99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_85.35100000039b99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430044
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG455.4612046E-419.998765
TCTGCGT600.003750381514.999078
ATTAGAC706.02881E-414.999073
GTACTTA600.003750381514.999071
AGGTAGT750.001016603213.9991326
CAGTGCT650.006380103513.8452969
GTATTAG1106.8897625E-613.6355181
GTATGTA800.001654247313.1241861
TATAGGA850.00260889112.3521752
GTTAATA1151.5749104E-411.7384021
ATATACC1551.5367423E-611.6121834
ATAGGGT1059.694233E-411.4278633
GAACAGT1059.694233E-411.4278636
TCTATTG1202.3389707E-411.2493033
ACTATGA1355.6317647E-511.1104228
TACGGTG950.00598903511.0519477
GAATATA1951.8838364E-710.7685621
GTATAGA1000.00876612110.499351
AACTATG1150.002071279610.4341357
GTATATA1809.206353E-69.999381
Produced by FastQC (version 0.11.2)