FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_84.35100000039b56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_84.35100000039b56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710339
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCC908.574625E-716.66600212
GTCCGGC908.574625E-716.66600211
CCCGCCC653.435348E-416.15320217
AGTCCCC600.003753036714.9994028
CCCCTAA600.003753036714.9994021
CCGGCCC809.7471224E-514.99940213
GGCCCGC706.0346624E-414.99940215
GCCCGCC706.0346624E-414.99940216
CGGCCCG851.6326121E-414.11708314
TAGGTAG851.6326121E-414.1170835
CAGCGTC800.001655820513.1244772
GCTACAC800.001655820513.1244773
AGTCCGG1006.445786E-411.99952110
GCGTCAG900.004005701311.6662024
GTATTAT3500.011.5709671
GTCCTGA1059.7060535E-411.4281151
GAACTAC950.005994566711.0521916
TAAGAGT950.005994566711.0521914
TGGACAG1100.001431991110.9086565
GTATTAA2156.428854E-810.4646991
Produced by FastQC (version 0.11.2)