FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_83.35100000039b05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_83.35100000039b05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824096
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGCC402.4679364E-422.4983168
TACCCGG1002.568493E-615.00160822
GCGTGTT706.037716E-414.998878511
GAGCCGA600.003754589714.99887711
CGGATCA1054.2614847E-614.28724526
CCGGATC902.648113E-413.33476425
GCAGCGT902.652197E-413.3323361
GCGTCAG902.652197E-413.3323364
ACCCGGA1201.6900174E-512.5013423
CCTGTAC850.002612645712.3520163
GGTTAGT1252.573533E-511.9991031
GTATTAA2257.094059E-1111.9991021
AGGCAGC900.00400767711.6657946
GTTTTAG1551.5426394E-611.6120341
CCTAATA2751.8189894E-1211.4536882
CTATACT2402.2191671E-1011.2491584
ACACCTG950.00599750411.0518055
AGTACAG1901.32286E-711.0518055
CTATGTA1501.3590634E-510.9991779
CCCGGAT1100.001430655410.9102624
Produced by FastQC (version 0.11.2)