FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_82.35100000039ac0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_82.35100000039ac0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166465
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT350.002996533921.4272844
AGAGTGT400.006489922818.7488756
ACACTGA400.006489922818.7488756
TATCCTG400.006489922818.7488755
CTGATCT600.003737481514.9990999
TCTTATA750.001011995513.9991592
TACACAT650.006358382313.8453237
TATTATA1106.8290738E-613.6355452
GTTTTAT800.001646816413.1242111
ATCATAG800.001646816413.1242111
ATTATAG900.003984315311.6659663
ATATAAT1355.5837496E-511.1104441
GTATAAA950.00596285611.0519671
TCATATA950.00596285611.0519672
TATAAAA1000.00872815510.4993692
Produced by FastQC (version 0.11.2)