FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_81.35100000039a8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_81.35100000039a8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356219
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG752.0008883E-1023.9973055
TATTAGG1053.092282E-1119.9977552
TTAAGCT559.5639705E-519.0887664
TAGTCAG601.8627195E-417.4980359
CCCTTAT550.00209938916.36181
ATTAGGT1306.4392225E-1016.1520333
ACTATGC859.177427E-615.8805698
GTATTAG1052.467059E-715.7125221
TAATACT1503.0195224E-1014.9983164
CTAATAC901.5771475E-514.9983163
CCTAATA1054.254807E-614.284112
AGAACTG851.6300098E-414.1160615
GACATAG650.006361613513.85043130
AGACATA650.006367535313.84848729
ACAGTGT650.006379393413.8445998
TTAGCTC650.006379393413.8445994
CTACACT650.006379393413.8445994
TACTATG1004.2588228E-513.4984847
CAGATGA1251.7688417E-613.19851813
CTATAAC800.001653902313.1235262
Produced by FastQC (version 0.11.2)