FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_80.35100000039a4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_80.35100000039a4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244521
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG300.001225205124.9950584
GTATAGG601.8609196E-417.496541
ACTAAGG653.4279554E-416.1506523
GTATATG653.4279554E-416.1506521
TAATACT706.0218276E-414.9970354
ATAACAC750.001015441313.9972333
GTATAGA650.006375455313.8434161
GTATATA1106.8703157E-613.6336681
TCTATAT902.643018E-413.3306971
CATAAAC1151.5715731E-411.7368113
TATATAA1100.00142805510.9069352
TATACAG1100.00142805510.9069355
CATATAT1000.00875677710.4979251
TTTATAT1000.00875677710.4979253
ATATATA1851.2696517E-59.7278062
Produced by FastQC (version 0.11.2)