FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_79.351000000399f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_79.351000000399f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673818
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCT350.003005999421.4271939
GGTAGTC1106.002665E-1119.0896827
GCCGCGT651.6430708E-518.4630925
TAGGTAG1151.1277734E-1018.2596955
TTAGGTA1450.017.5850754
TAAGACT653.4356606E-416.1528054
GTATTAG2150.016.0454791
CTAATAC1501.8189894E-1115.9989723
ATTAGGT1351.1041266E-915.5545563
TATTAGG1750.015.4275792
GGATAAG706.03521E-414.9990361
GGACAGC901.5805832E-514.9990366
TAGTCAG1351.8497303E-814.4435169
CGCCGCG851.630696E-414.11883524
ATAGAAC1201.1210959E-613.7491163
GCTATCC1004.2679523E-513.4991323
TAGAACT1151.0920743E-513.042644
ACCGTCG1404.50058E-712.8563168
CCTAATA2001.2732926E-1012.749182
TATACGG954.185696E-412.6307665