FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_78.351000000399b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_78.351000000399b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217385
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAC809.76116E-514.99954816
GGACCGT1351.8564606E-814.4440116
ACCGCGC650.00638978813.8457378
CCCCCGA902.6543613E-413.33293215
CCGACCG902.6543613E-413.33293218
CTAATAC7150.013.2163863
GCCCCCG1056.757167E-512.85675514
GTATTAG5250.011.1425231
GCCGCGT1653.2717708E-610.90999325
CTACCCC1653.2761945E-610.9087634
CCGTCCC1408.181679E-510.71420522
CTAGGAC1408.183552E-510.71396353
TTAGACT3950.010.6325924
ACTATGC6500.010.6150668
AGCGCGC1000.00877455210.50110429
ACTGGTC3009.094947E-1210.4996848
TAATACT9850.010.3550184
TCACACA4350.010.34591730
CGCCGCG1756.6163047E-610.2858724
AATACTA8750.010.2854045
Produced by FastQC (version 0.11.2)