FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_77.35100000039971.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_77.35100000039971.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543528
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGT400.006513919718.7487854
GTGCGCG600.003755506614.9995159
AGCGCAC1151.579547E-411.7387510
CCCCCGC900.004008149711.66666621
CCGGATC1951.52977E-811.53958325
TAGGTAG1951.53359E-811.5377145
TATACTG6450.011.394615
ACTATGC4000.011.2496368
GTACTAG2002.6859925E-710.50033951
TGGGGGG1150.00207656110.4341075
CCCGGAT1901.5764035E-610.26415524
ACCCGGA2053.7711106E-710.24423523
CCCGCGG1200.002954045310.00032423
AGGTAGC1200.00295563189.9996777
AGTCCGA1200.00295563189.99967710
TACCCGG2003.041192E-69.75031622
ACAGTGT2803.2323442E-99.6425458
TACTATG5150.09.611347
GTCCGGC1250.00414272039.599689511
TTAAGGT3306.002665E-119.5448364
Produced by FastQC (version 0.11.2)