FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_76.35100000039921.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_76.35100000039921.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407396
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGC400.006504526418.7494623
GGTGCGA500.001107851218.00169428
TGCGACG550.002097190816.36517730
ACTAGAC859.176614E-615.8818993
CTTACTC706.0263183E-414.9995713
CTAGACT600.003749097714.999574
CTAGACC750.001016185213.9995994
ACGGTGT750.001016185213.9995998
CCATGCT650.006377933513.8457579
GTATTAA1302.7468777E-612.6919431
TATGCAA1201.687419E-512.49964110
GGCAACA850.002607829912.35258811
CACGCAG850.002607829912.35258816
CTATGCA1101.0399896E-412.2723769
TATACAG2451.8189894E-1212.2445485
TATACGG1151.5740306E-411.7387945
TTACTCA900.00400035311.6663324
ACAGGCA900.00400035311.6663328
TCTTACT950.005986632311.0523152
CCTTAGA1100.001429584110.9087782
Produced by FastQC (version 0.11.2)