FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n01_ntr_75.351000000398ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n01_ntr_75.351000000398ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961558
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC2553.6379788E-1211.7634543
ACCGCGA900.004012821711.66542612
CAGAGCG900.004012821711.6654269
TAGGACT2301.3169483E-911.0857774
TCTAAGA4100.010.6086272
CTAGGCC1000.00878949610.4988824
TACACTG6900.09.9989375
TGGGCGA1901.7604818E-59.47267610
GTATATA19600.09.10617351
TATACTG10900.09.0816035
ACTGCCC1500.00182624048.9990438
ACCGAGC1500.00182624048.9990438
GCGCCAC1500.00182624048.99904313
CTAATAC6350.08.9754243
GTATTAA8650.08.842991
GTCTAGG1705.77919E-48.8225911
GTATAGA8500.08.8225911
TTAGGAC2403.2382395E-68.7490693
ACTGATC5350.08.6906648
AGGACAG2803.22083E-78.5705175
Produced by FastQC (version 0.11.2)